利用线性DNA供体进行预组装可以实现大片段基因组插入
https://www.nature.com/articles/s41586-026-10460-4 靶向插入大片段DNA在基因组工程和基因治疗领域具有广阔的应用前景 1,2 。双引物编辑引导RNA（Twin Prime Editing guide RNA）能够实现相对较大的插入
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